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蛋白质研究 >> 蛋白电泳 >> 4×SDS-PAGE分离胶缓冲液 |
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货 号: |
P0350 |
名 称: |
4×SDS-PAGE分离胶缓冲液 |
规 格: |
100ml/500ml |
价 格: |
60.00/180.00 |
CAS #: |
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4×SDS-PAGE分离胶缓冲液(PH=8.8)
含 1.5MTRIS-HCl PH8.8 0.4% SDS
如配制10ml分离胶,可加入此试剂2.5ml,不必再加5MTRIS-HCl 和SDS。
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TBST |
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应用举例:
|
分离胶12% |
分离胶10% |
分离胶8% |
浓缩胶3% |
总体积 |
10ml |
10ml |
10ml |
5ml |
4XSDS-PAGE分离胶缓冲液(PH=8.8) |
2.5ml |
2.5ml |
2.5ml |
0 |
4XSDS-PAGE浓缩胶缓冲液(PH=6.8) |
0 |
0 |
0 |
1.25 |
30%丙烯酰胺(29:1) |
4ml |
3.3 |
2.7 |
0.5 |
ddH2O |
3.4ml |
4.1ml |
4.7ml |
3.2ml |
10%过硫酸铵 |
100ul |
100ul |
100ul |
75ul |
TEMED |
10ul |
10ul |
10ul |
7.5ul |
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点击数:4414 修改时间:2010/8/19 【打印此页】 【关闭】 |
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