说明：DNA病毒的抑制剂。在免疫组化中的应用：BrdU标记细胞免疫组化染色(SP法)，BrdU(溴化脱氧尿嘧啶核苷)可以在体内和体外掺入到处于S期的细胞所合成的DNA链中，以标记DNA。
别名：5-溴尿嘧啶-2’-脱氧核苷；5-溴-2’-脱氧尿核苷；5-Bromo-2′-deoxyuridine，5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil5-Bromouracil deoxyriboside；BUdR；5-BrdU
分子式：C9H11BrN2O5
分子量：307.10
CAS＃：59-14-3
外观：白色或类白色粉末
特性：纯度：≥99% (HPLC) 熔点：191-194 ℃
溶解性：溶于水（参考浓度10mg/ml），也可溶于DMF
DMSO（参考浓度50-100mg/ml）。
注意事项：该品对肌体有不可逆损伤的可能性。使用时应穿防护服和戴手套。应避免吸入本品的粉尘。
储存条件：-20℃
BrdU相关文献（注意：并不代表本产品可全部用于以下实验）
1. PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines
2. Sequence of DNA replication in 277 R-and Q-bands of human chromosomes using a BrdU treatment
3. BrdU--Hoechst flow cytometry: A unique tool for quantitative cell cycle analysis* 1
4. Haematopoietic stem cells do not asymmetrically segregate chromosomes or retain BrdU
5. An avian muscle factor related to MyoD1 activates muscle-specific promoters in nonmuscle cells of different germ-layer origin and in BrdU-treated myoblasts.
6. In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse somatic and meiotic cells
7. BrdU-33258 Hoechst analysis of DNA replication in human lymphocytes with supernumerary or structurally abnormal X chromosomes
8. Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67 (MIB-5) immunohistochemistry and in situ hybridization for histone mRNA
9. BrdU immunohistochemistry for studying adult neurogenesis: paradigms, pitfalls, limitations, and validation
10. Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique
11. Increased number of BrdU-labeled neurons in the rostral migratory stream of the estrous prairie vole
12. Heritable fragile sites on human chromosomes. V. A new class of fragile site requiring BrdU for expression.
13. Monitoring S phase progression globally and locally using BrdU incorporation in TK+ yeast strains
14. Reinitiation of cellular DNA synthesis in BrdU‐selected nondividing senescent WI‐38 cells by simian virus 40 infection
15. Normal cycling patterns of hematopoietic stem cell subpopulations: an assay using long-term in vivo BrdU infusion
16. … the Selective Measurement of the Radiosensitivity of Quiescent Cells in Solid Tumors: Combination of Immunofluorescence Staining to BrdU and Micronucleus Assay
17. Analysis of exchanges in differentially stained meiotic chromosomes of Locusta migratoria after BrdU-substitution and FPG staining
18. Labelling of DNA and differential sister chromatid staining after BrdU treatment in vivo
19. Cell proliferation in the gastrulating chick embryo: a study using BrdU incorporation and PCNA localization |
